Part:BBa_K4628048

50Spro-miniSOG

This phototoxic protein, derived from LOV protein, requires light induction and produces toxic substances under blue light excitation to kill bacteria^[1]. It also emits green fluorescence for easy observation. To facilitate the use of this protein in Agrobacterium, its codon has been optimized.

Usage and Biology

In our study, we coupled the miniSOG protein with the 50Spro promoter and terminator BBa_B0015. These elements were cloned into reporter plasmids and subsequently transformed into Agrobacterium tumefaciens GV3101 for further examination.

Result：

our plate light experiment demonstrated a noticeable reduction of engineered bacteria under natural light irradiation, regardless of whether the conditions were sunny or cloudy.

FIG. Results of light experiments of Agrobacterium with empty plasmid V.S. miniSOG

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 517
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 517
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 517
  
• 1000
  COMPATIBLE WITH RFC[1000]